Development of micropropagation method by inducing embryogenic totipotent cells from non-pollen Cryptomeria

研究了无花粉柳杉（Cryptomeria sugi）无性系的离体培养技术。在细胞分裂素中，玉米素诱导芽的效果较好。雄性不育无性系福岛5号在含玉米素的培养基中诱导出的芽数比含BAP的培养基中多。芽在含有5 g/l活性炭的培养基1/2 LP上发育。在含有23.8 μM (5 mg/l) Cl-IAA和0.044 μM (0.01 mg/l) BAP的RIM培养基上生根。这些材料将用于杉木雄性不育的微繁，以减少空气中的花粉。

We developed the micropropagation technique using in vitro culture of non-pollen Cryptomeria (sugi) clones. Among the cytokinin tested, zeatin was relatively effective for bud induction. More buds were induced in the medium containing zeatin than that containing BAP with male sterile clone Fukushima 5. Shoots were developed from buds on the medium 1/2 LP containing 5 g/l activated charcoal. Shoots were rooted on the RIM medium containing 23.8 μM (5 mg/l) Cl-IAA and 0.044 μM (0.01 mg/l) BAP. These materials will be used for micropropagation of male sterile sugi cedar in order to reduce its pollen in the air.