Fundamental research on the development of high sensitive detection of target molecules by the use of self-assembled phospholipid layer possessing fluidity

我们旨在利用具有流动性的自组装磷脂层表面形成轮烷结构，开发一种新的高灵敏度的靶分子检测方法。众所周知，轮烷结构是由环糊精（CD）和聚乙二醇（PEG）在水中混合形成的，其中许多环糊精单元是由PEG串成的。将含有Cy3或Cy5诱导荧光共振能量转移（FRET）的两种CD衍生物固定在自组装磷脂层表面。观察到在含有Cy3或Cy5的CD膜固定化衍生物中加入PEG水溶液后，由于形成轮烷结构，荧光强度发生了变化。希望该方法能开发出高灵敏度的聚乙二醇检测方法。

We aimed to develop the novel high sensitive detection method of target molecules utilizing the formation of rotaxane structure on the surface of self-assembled phospholipid layer possessing fluidity. It is well-known that the rotaxane structure is formed by mixing cyclodextrin (CD) and polyethylene glycol (PEG) in water, in which many CD units are threaded by PEG. Two kinds of CD derivatives, which possessed Cy3 or Cy5 to induce the fluorescence resonance energy transfer (FRET), were immobilized on the surface of self-assembled phospholipid layer. It was observed that the fluorescence intensity changed by adding PEG aqueous solution to the film immobilizing CD derivatives possessing Cy3 or Cy5 due to the formation of rotaxane structure. It is hoped that the high sensitive detection of PEG might be developed by this method.