Development of selective chemiluminescence assay for biologicalquinones and its application to clinical investigation

醌类化合物在线粒体电子传递链和血液凝固等生物学功能中具有重要作用。另一方面，醌类被认为是一类毒素，它可以通过与蛋白质和DNA的共价结合，或通过生物系统中的氧化还原循环产生活性氧，对生命健康造成各种危害。本研究基于醌类化合物的特异性光反应和氧化还原反应，建立了灵敏、选择性强的生物醌类化合物的检测方法，并将其应用于生物样品，以评价所建立的方法在临床研究中的实用性。

Quinones are interesting compounds which have several important roles in biological functions such as mitochondrial electron transport chain and blood coagulation. On the other hand, quinones are regarded as a class of toxins which can cause a variety of hazardous effects on living health by the covalent binding to the protein and DNA, or the generation of reactive oxygen species through the redox cycle in biological system. In this study, sensitive and selective determination methods for biological quinones were developed based on the specific photoreaction and redox reaction of quinone and applied to biological samples to evaluate the usefulness of the developed method in clinical investigation.