CXCL14/BRAK is a multifunctional tumor suppressor

目的：研究趋化因子Brak/CXCL14转基因(TG)小鼠对肿瘤的发生、生长和转移是否具有抵抗力。方法：给野生型C57BL/6(Wt)或Tg小鼠腹腔注射12 mg/kg体重的偶氮甲烷，然后在饮用水中间歇加入3%葡聚糖硫酸钠。我们还将肿瘤细胞注射到小鼠的皮下或尾静脉。结果：Wt小鼠和Tg小鼠体重无明显差异，但Wt小鼠结直肠癌的发生率是Tg小鼠的近10倍(P&lt；0.001)。移植瘤大小和肺转移灶数目，试验组均明显低于对照组。注射黑色素瘤细胞的Tg小鼠存活率明显高于Wt小鼠。结论：BRAK/CXCL14对肿瘤的发生、生长和转移均有抵抗作用，提示Brak/CXCL14具有多种抑瘤作用。

Objectives: The aim of this study was to determine whether or not chemokine BRAK/ CXCL14 transgenic (Tg) mice would show resistance to carcinogenesis, tumor growth and tumor metastasis. Methods: Wild type C57BL/6 (Wt) or Tg mice were injected intraperitoneally with 12 mg/kg body weight azoxymethane followed by intermittent addition of 3 % dextran sodium sulfate in the drinking water. We also injected tumor cells subcutaneously or via a tail vein of the mice. Results: No difference of body weight was observed between Wt and Tg mice but the rate of colorectal cancer was nearly ten times higher (P<0.001) in Wt mice than in Tg ones. Size of transplanted tumors and the number of metastatic foci on the lung of the animals were always significantly lower in the Tg that those in the Wt. Survival rates of the melanoma cell injected Tg mice were significantly higher than those of Wt. Conclusion: The Tg mice showed resistance to carcinogenesis, tumor growth and tumor cell metastasis, indicating BRAK/ CXCL14 has multifunctional effects on tumor suppression.