Involvement of Polycomb group genes in cell line property of human ES and iPS cells

在本研究中，我们产生了自发分化，然后利用DNA微阵列分析了自发分化过程中基因表达的整体变化。通过对四个人类多能干细胞系进行基因芯片分析，我们发现在未分化状态崩溃的过程中，一些基因普遍上调或下调。此外，我们还利用CHIP-SEQ和CHIP-QPCR分析了多梳基团在未分化状态崩溃过程中的作用。

In this study, we produced the spontaneous differentiation, and then analyzed global changes of gene expression during the spontaneous differentiation using DNA microarray. As results of the microarray analysis using four human pluripotent stem cell lines, we found that some genes were commonly up- or down-regulated during the collapse of undifferentiated state. Furthermore, we analyzed the role of polycomb group during the collapse of undifferentiated state by ChIP-seq and ChIP-QPCR.