A general survey for teaching-aid kit design of biological teaching-materials and their apparatus by sterile condition

概述了生物学教材和仪器的设计概况，重点介绍了植物繁殖过程实验。随后，组装并设计了用于生物教学的体外试剂盒。本文报道了两种蕨类植物Ceratopteris richardii和海金沙Lygodoium japonicum Sw.用于体外培养。用常规技术灭菌的孢子和随后干燥的孢子用预灭菌的1/2 Murashige和Skoog培养基的盐沉降到在容器顶部安装有ED灭菌的注射器过滤器的聚苯乙烯容器中。用放大镜或双筒望远镜贴在培养容器底部，可以很容易地观察到发育中的配子体（颈卵器、精子器和精子）。用银杏雌株幼种子的花粉管（精母细胞）悬滴培养法，对银杏精子进行了巧妙的观察。

A general survey for design of biological teaching-materials and their apparatus were especially experimented on reproduction process in plant. Subsequently, an in vitro kit was assembled and designed for biological teaching. Two species of fern (Ceratopteris richardii,and Lygodoium japonicum Sw.)were used for in vitro culture. The spores sterilized with routine techniques and subsequently desiccated spores were settled with pre-sterilized 1/2 Murashige and Skoog medium’s salts into the polystyrene vessel installed with ED-sterilized syringe filter on top portion of the vessel. The developing gametophyte (archegonium, antheridium, and sperm) can easily observe attaching the bottom of culture vessel with a magnifier or binocular. Sperm of Ginkgo bilobawere skillfully observed with hanging-drop culture method of pollen tube (spermatocyte) derived from young female seeds.