Establishment of cancer therapytargeted for DNA dependent protein kinase and investigation of its predictive factor

NK314是DNA依赖性蛋白激酶（DNA- pk）和拓扑异构酶i α的双重抑制剂，能有效抑制成人T细胞白血病和肺癌细胞系的细胞生长。抑制作用是通过增强DNA双链断裂和延长DNA修复抑制作用介导的。对于DNA-PK抑制剂的预测因素，RNA结合蛋白hnRNP B1的蛋白水平与DNA-PKcs的蛋白水平密切相关。然而，肺癌细胞生长的抑制作用与DNA-PKcs和hnRNP B1蛋白水平无统计学相关性。需要进一步调查以寻找预测因素。

NK314, a dual inhibitor of DNA dependent protein kinase (DNA-PK) and topoisomerase IIαpotently inhibited cell growth of adult T cell leukemia and lung cancer cell lines. The inhibitory effect was mediated through enhancement of DNA double strand breaks and prolonged inhibitory effect ofDNA repair. As for the predictive factors for DNA-PK inhibitors, protein level of hnRNP B1, an RNA binding protein, was strongly associated with that of DNA-PKcs. However, the inhibitory effect of cell growth of lung cancer cells was not statistically associated with protein levels of DNA-PKcs nor hnRNP B1. Further investigation for seeking the predictive factors would be needed.