Development of a biomarker for small intestinal mucosal injury following chemotherapy for gastric cancer and establishment of new method to prevent gastrointestinal adverse event

为了明确化疗后胃肠道不良事件(主要是腹泻)和小肠的形态变化，我们用光镜、免疫组织化学(增殖细胞核抗原和Ki67)和扫描电子显微镜研究了DAO活性与小肠形态变化的关系。此外，还研究了可溶性膳食纤维对小鼠小肠损伤的预防作用。结论：1)DAO活性可以反映化疗后的小肠损伤；2)可溶性膳食纤维可能对改善化疗后的小肠损伤有一定作用。

To clarify the gastro-intestinal adverse event (mainly diarrhea) and morphological change of the small intestine after chemotherapy, we investigate the correlation between DAO activity and morphological change of small intestine by light microscopy, immuno-histochemistry (for PCNA and Ki67) and scanning electron microscopy. We also investigated that soluble dietary fiber could prevent the injury for small intestine. The conclusions are 1) DAO activity can indicate the small intestine injury after chemotherapy, and 2) soluble dietary fiber may have an effect to improve the small intestine injury.