Development of an automated chromatographic determination method of surfactants using microfluidic chip detector

采用嵌入AS离子选择电极（AS—ISE）作为检测器的微流控聚合物芯片，研制了一种测定阴离子表面活性剂（AS）的序贯注射分析（SIA）系统。在SIA系统的最优流动条件下，在3×10-6M~1×10-3 M mol dm-3浓度范围内，AS—ISE显示出良好的峰高与对数量级浓度之间的线性关系，其Nernstian斜率为60.0 mV 10- 1。本系统的样品通量约为20个样品h-1。所提出的SIA系统被应用于环境水样（如河水）中砷含量的测定。

A sequential injection analysis (SIA) system for the determination of anionic surfactant (AS) using a microfluidic polymer chip with an embedded AS ion-selective electrode (AS--ISE) as a detector was developed. Under the optimal flow conditions of the SIA system, the AS--ISE showed a good linear relationship between peak heights and logarithmic concentrations of ASion with a Nernstian slope of 60.0 mV decade-1in a concentration range from 3×10-6M～1×10-3 M mol dm-3. Sample throughput of the present system for the determination of ASion was ca. 20 samples h-1. The proposed SIA system was applied to the determination of the level of ASion in environmental water samples, such as river water.