Modification of chloroplast proteins with reactive aldehydes: its implication in the plant response to oxidative stress

本研究获得了以下有关过氧化脂质衍生的活性醛（RAL）在植物逆境响应中的作用的结果：（1）鉴定了铝诱导的根氧化损伤和强光诱导的叶光氧化损伤的RAL种类。(2)光依赖性的有机过氧化物的光系统II，缺乏水氧化复合体的供体侧的形成被证明。(3)在NaCl胁迫的植物中，RAL对蛋白质的修饰增加。确定了修改后的目标。(4)磷酸核酮糖激酶中对丙烯醛敏感性具有首要重要性的靶氨基酸不是氧化还原调节的Cys。

We obtained following results about the action of lipid peroxide-derived reactive aldehydes (RAL) in plant responses to environmental stress:(1) The RAL species responsible for the aluminum-induced oxidative injury in roots and those for the strong-light-induced photooxidative injury in leaves identified.(2) Light-dependent formation of organic peroxides on the donor side of photosystem II that lacked the water-oxidizing complex was proven.(3) In NaCl-stressed plants, modification of proteins with RAL were increased. The modified targets were identified.(4) The target amino acid(s) in phosphoribulokinase that have primary importance in the sensitivity to acrolein was not the redox-regulated Cys.