Genetic analysis of the haploid strain in the potato late blight pathogen Phytophthora infestans

马铃薯晚疫病菌Phytophthora infestans一直被认为是二倍体或更高倍性的，但最近我们发现了一个单倍体分离物。本研究对该菌株进行了遗传分析，包括：（1）基于核基因和线粒体基因的分子系统发育分析;（2）核DNA含量和基因组稳定性的流式细胞术分析;（3）体细胞核和染色体的显微观察;（4）生长特性;（5）营养缺陷型突变体的产生;（6）转化方法的建立。

Although the potato late blight pathogen, Phytophthora infestans, has long been believed to be diploid or of higher level of ploidy, we discovered a haploid isolate recently. In this study, we carried out genetic analyses on this isolate, including (1)molecular phylogenetic analysis based on nuclear and mitochondrial genes; (2) flow cytometric analysis of nuclear DNA content and stability of genome; (3)microscopic observation on the somatic nuclei and chromosomes; (4)growth characteristics; (5)production of auxotrophic mutants; (6)establishment of transformation method.