Screening of protein-protein interactions between viruses and the brown planthopper, Nilaparvata lugens.

许多植物病毒病都是由半翅目昆虫传播的。这些半翅目昆虫体型较小(1-4毫米)，但繁殖能力很高。这一特点使昆虫对杀虫剂产生了耐受性。近年来，亚洲稻飞虱对一些农药产生了抗药性，水稻病毒病成为严重的问题。为了防止这些昆虫传播病毒，我们必须了解病毒如何利用媒介昆虫中的分子通过它们传播。然而，昆虫与传播病毒之间的分子相互作用还没有得到很好的了解。为了揭示这些相互作用，我们研究了褐飞虱、褐飞虱和由该昆虫传播的病毒之间的蛋白质-蛋白质相互作用。酵母双杂交检测到许多可能与昆虫体内相互作用的飞虱候选蛋白。

Many viral diseases of plants are transmitted by hemipterans. These hemipteran insects are small in size (1-4 mm) but their reproductive capacity is very high. This characteristic allows insects to get tolerance for pesticides. Recently, planthoppers acquired resistance against some pesticides and virus diseases of rice plants became serious problem in Asia. To prevent virus transmission by these insects, we have to understand how viruses utilize molecules in vector insects to be transmitted by them. However, molecular interactions between insects and the transmitted viruses are not understood well. To reveal these interactions, we searched protein-protein interactions between the brown planthopper, Nilaparvata lugens, and viruses transmitted by the insect. Yeast two hybrid analyses detected many planthopper candidate proteins which may be interact in insect body.