Development of the nucleic acid aptamer for anti HIV drug

艾滋病(获得性免疫缺陷综合征)是人类免疫缺陷病毒(HIV)引起的传染病。艾滋病是由于免疫缺陷而发展为机会性感染或恶性肿瘤的综合征。由于艾滋病毒很容易引起变异，出现抗药性病毒是个严重的问题。因此，需要从不同的角度开发抗HIV新药。在本研究中，我试图开发一种与HIV蛋白酶特异结合的核酸适配子，并将其应用于一种抗HIV药物。利用纯化的酶和SELEX方法，提出了开发与HIV酶有亲和力的适体的可能性。

AIDS (acquired immunodeficiency syndrome) is infectious disease caused by HIV (human immunodeficiency virus). AIDS is the syndrome that developed the opportunistic infection or malignant tumor by immunodeficiency. Because HIV is easy to cause mutations, the appearance of the drug-resistant virus is the serious problem. Thus, the development of the new anti HIV drug from a different point of view is demanded.In this research, I tried to develop a nucleic acid aptamer, which specifically binds to HIV protease, and apply it to an anti HIV drug.I could construct a wild type and 2 kinds of mutant HIV proteases in this research. And the possibility of the development of aptamer, which has affinity to HIV proteases, was suggested by using purified enzymes and SELEX method.