Biological implication of microRNAs in tumorigenesis, tumor development, and progression ? analysis with in situ hybridization using histopathological sections

尽管我们在本研究中检查了各种肿瘤，包括食管癌（鳞状细胞癌）、胃癌（腺癌）、卵巢癌（腺癌）和肝细胞癌，但除食管癌外，没有发现microRNA与其靶向特异性蛋白之间存在明显的生物学相互关系。我们认为，未能检测到相互关系的中心原因是ISH探针的恶化和供应它们的公司的意外变化。相反，我们发现microRNA 199a、Brm和Egr 1在食管癌中构建了双负饲料，表明它们在食管癌的发生、发展和进展过程中相互关联。但与肿瘤分期无特殊关系。我们正在继续研究其他上皮性和非上皮性肿瘤以及各种癌前病变，因为这种研究方法非常独特。

Although we examined various tumors including esophageal cancer (squamous cell carcinoma), gastric cancer (adenocarcinoma), ovarian cancer (adenocarcinoma), and hepatocellular carcinoma in this study, no apparent biological interrelationship between microRNAs and their targeted specific proteins was revealed except esophageal cancer. We supposed that the central reason for the failure to detect the interrelationship was deterioration of ISH probes and unexpected change of company that supplied them. On the contrary, we found that microRNA 199a, Brm, and Egr1 constructed double-negative feed in esophageal cancer, suggesting that they were mutually related in the process of esophageal carcinogenesis, cancer development, and its progression. But no special relationship was found between them and tumor stage. We are continuously studying about other tumors both epithelial and nonepithelial and various precancerous lesions because this research method is very unique.