Analysis of prognostic predictor for papillary thyroid carcinoma

近3年来，应用细针抽吸(FNA)标本对甲状腺乳头状癌进行了BRAF基因突变分析。我们利用FNA样本研究了BRAF突变(T1796A)与日本人PTC临床恶性程度的关系。在手术切除的PTC组织中，BRAF突变(T1796A)为阳性或阴性，直接测序法显示BRAF突变阳性的PTC存在异质性等位基因。我们将排除血细胞、间充质细胞和内皮细胞等野生型等位基因，进一步研究其遗传异质性。

Analysis of BRAF mutation in papillary thyroid carcinoma using Fine-needle aspiration (FNA) sample is established in recent 3 years. We showed the relationship between BRAF mutation (T1796A) and clinical malignancy of Japanese PTC using FNA sample. In surgical PTC tissue,BRAF mutation (T1796A) is positive or negative, the direct sequence method revealed PTC with positive BRAF mutation had heterogeneous alleles. We are going to exclude wild type allele such as blood cells, mesenchymal cells and endothelial cells, further investigate the genetic heterogeneity .