Are platelet-derived / endothelial cell-derived microparticles risks for cardiovascular events in general population?

我们试图研究在Tanushimaru研究中登记的健康普通受试者中血小板源性MP（PMPs）和内皮源性MP（EMP）的临床意义。在2，100名受试者中获得富含PdR/EMP的部分并保持冷冻。本研究参照目前广泛使用的流式细胞仪的方法，采用灵敏度高、特异性强的新型流式细胞仪进行PGEX/EMP采样和流式细胞术检测。然而，PdR和EMP测量缺乏重现性。因此，我们试图优化流式细胞术程序。优化后，获得了可接受的重现性定量PdR测量，但我们还没有优化的定量EMP测量的程序。

We sought to investigate the clinical significance of platelet-derived MP (PDMP) and endothelium-derived MP (EMP) in healthy general subjects who were registered in the Tanushimaru study. PDMP-/EMP-rich fraction was obtained and kept freezed in 2,100 subjects. We performed PDMP/EMP sampling and flow-cytometry using new flow cytometric machine with high sensitivity and specificity, according to the widely used method which was done using old-type of flow cytometric machine. However, the PDMP and EMP measurements were lack in the reproducibility. Thus, we tried to optimized the flow cytometric procedure. After optimization, acceptable reproducibility of quantitative PDMP measurement was obtained, but we have not yet optimized the procedure of quantitative EMP measurement.