Identification of the marker for cholangiocarcinoma based on the data by proteomics and tissue microarray

我们用蛋白质组学方法对切除的胆管组织标本进行分析，在38个与正常胆管组织相比高表达的基因产物中检测到4个独特的蛋白质(肌动蛋白-1、肌动蛋白-4、DJ-1、组织蛋白酶B)。接下来，我们用Western blotting方法检测了这4种蛋白在19个胆管癌细胞系中的表达水平，并将其分为低表达细胞系和高表达细胞系。最后，我们利用组织芯片对连续96例胆管癌标本进行了免疫组织化学分析，在4种蛋白中，dj-1的高表达可作为预后良好的标志(p=0604)。

We analyzed resected specimens by the proteomics approach anddetected 4 unique proteins (actinin-1,actinin-4,DJ-1,cathepsin B) out of 38 higher gene expression products compared with normal bile duct tissue. Next,we evaluated theexpression level of these 4 proteins by Western blotting method among 19 bile ductcarcinoma cell lines and characterized them into low expression cell lines and highexpression cell lines. We Finally, we performed immunohistochemical analysisutilizing tissue micro array of the consecutive 96 resected specimens of bile ductcarcinoma Among 4 proteins, high expression of DJ-1 could be a marker to suggestgood prognosis (p=0604)