Therapeutic effects of transplanted peripheral blood mononuclear cells which mobilized by G-CSF on spinal cord injury in mice

我们分析了由粒细胞集落刺激因子(G-CSF)动员的CD34+外周血细胞脊髓内移植可促进损伤脊髓血管生成，促进脊髓损伤后功能恢复。NOD-SCID小鼠造成挫伤致脊髓损伤。脊髓损伤后1周将G-CSF动员的人CD34+外周血干细胞(1.0×105)注入损伤脊髓内(CD34组)。同时给予G-CSF动员的人单核细胞分数(1.0×10~(-5))(M组)或生理盐水(对照组)。移植后6~8周，CD34组和M组小鼠后肢运动功能明显恢复(P<0.01)。检测到一定数量的移植细胞，直到四周，它们被分化为血管内皮细胞。结果表明，G-CSF动员的人外周血干细胞脊髓内移植可促进损伤脊髓血管生成，促进功能恢复。

We analyzed that intraspinal transplantation of CD34+ peripheral blood cells mobilized by granulocyte colony-stimulating factor (G-CSF) facilitates angiogenesis in injured spinal cord and promotes functional recovery after spinal cord injury (SCI). NOD-Scid mice were subjected to contusion-induced SCI. Human CD34+ peripheral blood stem cells (1.0x10^5) mobilized by G-CSF were injected into the injured spinal cords (CD34 group) one week after SCI. Human mononuclear cell fraction (1.0x10^5) mobilized by G-CSF (M group) or saline (Control group) were also administrated. Mice in CD34 and M groups showed significant locomotor recovery of hind limb motor function fromsix to eight weeks after the transplantation (p<0.01). A certain amount of transplanted cells were detected until four weeks, and they were differentiated into vascular endothelial cells. Those results indicate that intraspinal transplantation of human peripheral blood stem cells mobilized by G-CSF facilitates the angiogenesis in injured spinal cord and promotes functional recovery in mice.