Analysis of constriction mechanisms in response to oxygen in the ductus venosus and umbilical vessels-The role of sarcoplasmic reticulum and Rho kinases-

采用实时荧光定量PCR技术分析兔脐动静脉血管收缩/扩张相关基因的表达。RhoB和Rho激酶1在脐带血管中的表达水平分别高于RhoA和Rho激酶2。胎儿发育过程中，脐血管中Rho通路基因的表达水平相对稳定。随着胎儿发育，肌浆网（SR）中Ca^<2+>的调控/储存相关基因和收缩蛋白的表达增加。SR Ca^<2+>调节/储存蛋白基因的表达水平在这些脐带血管中存在差异。由于参与Ca^<2+>调控的SR蛋白的含量和组成在胎儿发育过程中有所不同，因此Ca^<2+>释放/储存的SR活性可能因脐带血管和发育阶段的不同而不同。

Gene expression related to vascular constriction/dilation in rabbit umbilical arteries and veins was analyzed by real-time quantitative PCR. Expression levels of RhoB and Rho kinase 1 in the umbilical vessels were higher than those of RhoA and Rho kinase 2, respectively. Expression levels of Rho pathway genes in umbilical vessels during fetal development were relatively stable. Gene expression related to the regulation/storage of Ca^<2+> in the sarcoplasmic reticulum(SR) and the contraction proteins was increased as fetal development. Expression levels of the SR Ca^<2+> regulation/storage protein genes varied among these umbilical vessels. Since the content and constitution of SR proteins involved in Ca^<2+> regulation varied during fetal development, the SR activity of the Ca^<2+> release/storage might be differ according to the umbilical vessel and the developmental stage.