OASIS, a CREB/ATF-family transcription factor, modulate transcription of C6ST1 gene and chondroitin sulfation

在中枢神经系统中，内质网应激与包括缺血性损伤和神经退行性疾病在内的一系列疾病有关。损伤皮质中OASIS的mRNA和蛋白水平均升高，且与反应性星形胶质细胞的表达密切相关。反应性星形胶质细胞是胶质瘢痕的主要细胞成分，包裹在病变部位，并产生抗再生分子，如硫酸软骨素蛋白多糖(CSPGs)。我们利用OASIS基因敲除小鼠分析了CS-糖胺聚糖合成酶的mRNA水平。软骨素6-磺基转移酶1(C6ST1)在野生型小鼠皮质损伤中表达增加，而在绿洲基因敲除小鼠中不表达。此外，OASIS结合第一内含子区域并促进C6ST1的转录。这些结果表明，OASIS可能通过转录C6ST1参与星形胶质细胞CSPG的产生。

In the central nerve system, ER stress is implicated in a wide range of disorders including ischemic injury and neurodegenerative disorder. Both mRNA and protein levels of OASIS were elevated in the injured cortex and correlated closely with those of reactive astrocyte. Reactive astrocyte, which constitute a major cellular component of glial scar, enmesh the lesion site and produce anti-regenerative molecules such as chondroitin sulphate proteoglycans(CSPGs). We have analyzed the mRNA levels of the CS-glycosaminoglycan synthesizing enzymes using OASIS knockout mice. Expression of chondroitin 6-sulfotransferase 1(C6ST1) is increased in cortical stub injury in wild type mice, but is not in OASIS knock out mice. Furthermore, OASIS bound to the first intron region and promoted transcription of C6ST1.These results suggest that OASIS may be involved in CSPG production through the transcription of C6ST1 in astrocytes.