Cellular and molecular biological analysis of the impact of drugs used during periorerative period on cellular oxygen metabolism

我们证明，由400 mg/dL葡萄糖引起的葡萄糖刺激的胰岛素分泌（GSIS）被异氟烷和七氟烷处理抑制。实验证据表明，格列本脲刺激的胰岛素分泌不受异氟烷或七氟烷的影响，并且异氟烷或七氟烷治疗可显著抑制葡萄糖诱导的细胞内ATP升高，因此，异氟烷和七氟烷可影响线粒体ETC并抑制GSIS。我们的发现可能揭示了挥发性麻醉药建立糖耐量的分子机制。

We demonstrated that Glucose-stimulated insulin secretion(GSIS) elicited by 400 mg/dL glucose was inhibited by treatment with isoflurane and sevoflurane. Taken together experimental evidence that glibenclamide-stimulated insulin secretion was not affected by either isoflurane or sevoflurane and that treatment with isoflurane or sevoflurane significantly suppress glucose-induced intracellular ATP elevation, it is suggested that isoflurane and sevoflurane affect mitochondria ETC and suppresses GSIS. Our finding may delineate the molecular mechanism behind the glucose tolerance established by volatile anesthetics.