Molecular biological analysis of otitis media with effusion

非典型流感嗜血杆菌（NTHi）是引起渗出性中耳炎的病原菌之一，可形成生物膜.我们用新的最小生物膜根除浓度（MBEC）方法测定了生物膜中临床分离株的NTHi对抗菌药物的敏感性。此外，我们还评估了抗菌药物暴露时间的影响，不同抗菌药物对NTHi生物膜的敏感性存在很大差异。β-内酰胺类药物对生物被膜中细菌的清除率和生物被膜体积的影响不大。但大环内酯类药物相对有效。在生物被膜形成初期，抗菌剂对生物被膜有抑制作用。然而，抗菌剂并没有减少成熟的生物膜。

Nontypable Haemophilus Influenzae(NTHi) is one of the causative pathogen of otitis media with effusion and form biofilm. We determined antimicrobial agents susceptibility of NTHi from clinical isolates in biofilm with the new minimal biofilm eradication concentration(MBEC) method. In addition, we have assessed the effect of antimicrobial exposure timing.There were large differences in susceptibility of NTHi biofilm between antimicrobial agents. Beta-lactams had little effect on eradication bacteria in biofilm and biofilm volume. However, macrolides were comparatively effective. Biofilm was inhibited by antimicrobial agents in the early stage of biofilm. However, antimicrobials did not decrease mature biofilm.