Development and clinical evaluation of soft tissue regeneration based on mechanobiology

本实验主要研究了LIPUS对GE 1细胞的作用。GE 1暴露于LIPUS 15 min，频率为3 MHz，功率为40 mW/cm 2。在LIPUS暴露后提取总RNA并通过定量PCR分析以检测CCN 2/CTGF，并且在LIPUS暴露后来自每个样品的总蛋白进行免疫印迹。与对照组相比，CCN 2/CTGF mRNA水平在LIPUS暴露后0和15 min显著升高。Western blotting分析显示LIPUS暴露后CCN 2/CTGF的强染色持续60 min。MAPK磷酸化在LIPUS暴露后30 min上调。我们的研究结果表明，LIPUS暴露通过MAPK信号通路增加CCN 2/CTGF。

We focused on the effects of LIPUS on GE1 cells in this study. GE1 were exposed to LIPUS for 15 min at 3 MHz frequency and 40 mW/cm2 power. Total RNA was extracted after LIPUS exposure and analyzed by quantitative PCR to detect CCN2/CTGF, and total protein from each sample after LIPUS exposure was immunoblotted. CCN2/CTGF mRNA levels were significantly greater at 0 and 15 min after LIPUS exposure, compared with the control. Western blotting analysis showed intense staining of CCN2/CTGF for 60 min after LIPUS exposure. Phosphorylation of MAPK was up-regulated at 30 min after LIPUS exposure. Our findings demonstrated that LIPUS exposure increases CCN2/CTGF via the MAPK signaling pathway.