Development of next-generation therapy for temporomandibular joint arthrosis by gene transfer technique using nanofullerene and ultrasonic wave

我们利用纳米富勒烯和超声波的基因转移技术开发了下一代颞下颌关节关节病的治疗方法。将HIG-82细胞接种到硅室中。将腔室连接到施加单轴正弦拉伸力的拉伸装置。ELISA法测定IL-1β、IL-1 ra、PGE 2的释放量。纳米富勒烯预处理抑制机械拉伸诱导的PGE 2合成。我们用声孔法将DNA-纳米富勒烯转染入HIG-82细胞，并检测转染效率。当用纳米富勒烯超声处理细胞时，转染效率显著增加。

We develop the next-generation therapy for temporomandibular joint arthrosis by gene transfer techmique using the nanofullerene and ultrasonic wave. HIG-82 cells were seeded into silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The amounts of IL-1β, IL-1ra, PGE2 released into the culture medium were determined using ELISA. Nanofullerene pretreatment suppressed PGE2 synthesis induced by mechanical stretch. We used sonoporation to transfect DNA-nanofullerene into HIG-82 cells and examined the transfection efficiency. Transfection efficiency was significantly increased when the cells were ultrasonicated with nanofullerene.