Analysis of promoter of human BRAK/CXCL14, a tumor suppressor

BRAK/CXCL 14是一种在头颈部鳞状细胞癌中不存在或以非常低的水平表达的趋化因子。我们将此基因作为分子靶向治疗。为了研究该基因表达的调控机制，我们确定了该基因表达的启动子基序。结果表明，TATA样序列TATTAA是刺激该基因在人鳞状上皮细胞中表达所必需的，AP-1结合序列和串联GC Box 3，4是刺激该基因在人鳞状上皮细胞中表达所必需的。

BRAK/CXCL14 is a chemokine that is absent from or expressed at very low levels in head and neck squamous cell carcinoma. We set to this gene as molecular target therapy. In order to study the regulatory mechanisms governing the expression of this gene, we determined the promoter motifs of the expression of this gene. The results of that, TATA-like sequence, TATTAA was essential for stimulating the expression of the gene and the AP-1 binding sequence and tandem GC Box3, 4 were necessary for stimulating the expression of the gene in human squamous epithelial cells.