Examination of intracellular localization and transport pathway of bone sialoprotein.

骨涎蛋白(BSP)被认为是骨钙化的重要蛋白质之一。为了阐明BSP对BSP的作用机制，我们研究了BSP蛋白在细胞内转录和转运途径的调控。在此，我们用骨形态发生蛋白2(BMP-2)刺激成肌细胞C2C12细胞后，我们发现Smad1与BSP启动子序列中的Hox结构域结合，Smad1和Runx2结合FRE结构域。我们还检测到C2C12细胞表达BSP蛋白以诱导钙化。现在，我们正在用电子显微镜观察细胞内的定位。

Bone sialoprotein(BSP) is expected one of the important protein for calcification of bone. In order to clear the mechanisms of it through BSP, we investigated regulation of BSP transcription and transport pathway of BSP proteins in cell. Here, after stimulation with bone morphogenetic protein 2(BMP-2) of C2C12 cells which were myoblast, we showed that Smad1 bound to HOX domain in BSP promoter sequence and that Smad1 and Runx2 bound to FRE domain. And also we detected that C2C12 cells expressed BSP protein to induce calcification. Now, we are observing intracellular localization in the cells by electron microscopy.