Structural analysis of H+-translocating inorganic pyrophosphatase

质子转运无机焦磷酸酶是一种以无机焦磷酸盐为底物的产电质子泵。该蛋白质家族的成员广泛分布于大多数植物中，但仅限于一些原生动物、细菌和古细菌。虽然它在膜转运系统中的重要性，它的焦磷酸水解和质子主动运输之间的耦合机制仍然是未知的。在这里，进行了植物来源的酶的结构研究。

Proton-translocating inorganic pyrophosphatase is an electrogenic proton pump that uses inorganic pyrophosphate as the substrate. The members of this protein family are widely distributed in most plants, but only some protozoa, bacteria, and archaebacteria. Although its importance in membrane transport systems, its coupling mechanism between the pyrophosphate hydrolysis and the active proton transport is still unknown. Here, structural study of the plant-derived enzyme was carried out.