An electron microscopic study of actin nucleation factors

肌动蛋白丝负责细胞的大范围运动和动态的构建和破坏。许多蛋白质调节肌动蛋白丝的动态。Arp2/3复合体和formin是其中的两种调节蛋白，它们在细胞中启动肌动蛋白丝的构建。为了了解开始构建的机制，我们与一个奥地利小组开发了细胞中肌动蛋白丝网络的结构分析程序，通过电子显微镜和图像分析确定了Arp2/3复合物在细胞中的分布和结构。我们还阐明了双胍只结合在肌动蛋白丝的末端，而不是一侧。现在我们正在分析数据，以确认绑定端是有刺端。

The actin filament is responsible for a wide range of movement of the cell and dynamic through construction and destruction. Many proteins regulate the dynamics of the actin filament. Arp2/3 complex and formin are ones of the regulatory proteins and they start the construction of the actin filament in the cell. To understand mechanism of starting construction, we developed structural analysis procedures of the actin filament network in the cell with an Austrian group, which determined distribution and structure of Arp2/3 complex in the cell by using electron microscopy and image analysis. We also elucidated that formin binds only to the ends of the actin filament, not the side. Now we are analyzing data to conform the bound end to formin is the barbed end.