Analysis of gene expression in the cells isolated by laser capture microdissection from different petal tissues

采用激光捕获显微分离技术从香石竹花瓣中分离出表皮、薄壁组织和维管束细胞，并对这些组织中的差异基因表达进行了研究。此外，通过基因芯片分析，揭示了香石竹花瓣弯曲区薄壁组织近轴面和远轴面基因表达的差异。编码脂质转运蛋白（DcLTP1）的基因在叶片表皮中表达量较高，而在弯曲区近轴侧的薄壁组织中表达量较高。

Cells from epidermis, parenchyma and vascular bundles were isolated from carnation petals by laser capture microdissection and differential gene expression among these petal tissues was demonstrated. In addition, differential gene expression between adaxial and abaxial sides of parenchyma in a bending zone of carnation petals was revealed by macroarray analysis. One gene encoding lipid transfer protein(DcLTP1) showed relatively high expression level in blade epidermis, while its expression was higher in the adaxial side of parenchyma from the bending zone.