Analysis of the origin and the mechanism of persistent infection of BmMLV

为了确定家蚕斑斑样病毒的来源，我们分析了BmMLV的寄主范围。将BmMLV接种于昆虫和哺乳动物来源的几个细胞系，分析BmMLV的增殖情况。我们发现只有家蚕来源的细胞系对BmMLV的持续感染是允许的。同时，将BmMLV阴性的BmVF细胞接种BmNPV。我们证实重组蛋白在BmNPV感染的BmVF细胞中得到了充分表达，并发生了适当的翻译后修饰。这些结果表明，BmMLV只在家蚕来源的细胞中复制，BmVF细胞适合作为杆状病毒表达载体系统。

To identify the origin of Bombyx mori macula-like virus, we analyzed the host range of BmMLV. Several insect-and mammalian-derived cell lines were inoculated with BmMLV and analyzed for the propagation of BmMLV. We showed that only B. mori-derived cell lines were permissive to BmMLV persistent infection. Also, BmMLV-negative BmVF cells were inoculated with BmNPV. We identified that the recombinant protein was fully expressed and suitable post-translational modification was occurred in BmNPV-infected BmVF cells. These data showed that BmMLV replicates only in B. mori-derived cells and BmVF cell line was suitable for baculovirus expression vector system.