Study on molecular mechanisms of trypanosome cell differentiation for developing novel preventives against the parasite

体外诱导刚果锥虫细胞由后循环向血流分化，并用2D-DGE分析细胞分化过程中蛋白质表达的变化。结果表明，诱导分化6h后，15种蛋白质表达增加，9种蛋白质表达下降1.5倍以上。诱导分化12h后，14种蛋白质表达增加，78种蛋白质表达下降2倍以上。

The cell differentiation from metacyclic form to bloodstream form of Trypanosoma congolense was induced in vitro, and the alternation of protein expression during the differentiation was analyzed by 2D-DIGE. The results showed that expressions of 15 proteins were increased but those of 9 proteins were decreased more than one and a half times after 6-hour induction of the differentiation. Meanwhile, expressions of 14 proteins were increased but those of 78 proteins were decreased more than twice after 12-hour induction of the differentiation.