Establishment of in vitro selection system using recent primary isolates, and a new efficient treatment for HIV

用抗HIV-1药物体外筛选获得遗传异质性的HIV-1原代分离株。在体外选择过程中，Env多样性显著减少。此外，这些药物选择变异具有与传代对照完全不同的Env序列。本研究首次利用体外选择方法探讨了抗HIV药物对含有高度多样化Env序列的大量HIV原代分离株遗传瓶颈的影响。体外选择可能是研究使用进入抑制剂联合抗逆转录病毒治疗的相容性的有用工具。

In vitro selection with anti-HIV-1 drugs were used to obtain genetically heterogeneous primary HIV-1 isolates. Env diversities significantly decreased during in vitro selection. Furthermore, these drugs-selected variants had completely different Env sequences from that in the passage control. This study is the first to explore the influence of anti-HIV drugs on genetic bottlenecks in bulk HIV primary isolates containing highly diverse Env sequences using in vitro selection methods. In vitro selection may be a useful tool for investigating the compatibility of combined anti-retroviral therapy using entry inhibitors.