New drug sensitivity test for leukemia mimicking bone marrow microenvironment in hypoxia

对白血病的治疗，需要准确的药敏试验来评价抗白血病药物。我们认为，模拟骨髓微环境的低氧细胞培养系统比常氧系统更有助于评估效果。在本研究中，我们发现低氧培养系统上调白血病干细胞中HIF蛋白的表达，并影响Notch、NF-kB和mTOR信号转导。为建立一种新的耐缺氧药敏试验方法奠定了基础。

For treatment of leukemia, it is necessary to assess antileukemia agent by precise drug sensitivity test. We assumed that cell culture system in hypoxia mimicking bone marrow microenvironment is useful for assess the effect than the system in normoxia. In this study, we found that hypoxia culture system upregurated HIF protein expression as well as affected Notch, NF-kB, and mTOR signaling in leukemia stem cells. We established a basis for a new drug sensitivity test in hypoxia.