Investigation of importance of existence of the primary cilia involved in viability of the corneal endothelium.

我们检查了美国眼库提供的用于角膜移植的供眼角膜内皮细胞，我们发现内皮细胞中约有5%的死亡细胞。我们认为，角膜移植后内皮细胞数量的减少可能与贮藏过程中死亡细胞的增加有关。另外，当兔角膜在角膜储存介质中保存4℃时，角膜内皮细胞中的原代纤毛消失，37℃恢复培养后重新出现。原纤毛的存在被认为与角膜内皮的活力有某种关系。

We examined corneal endothelia of donor eyes which were provided by the U.S. Eye-bank and were used for corneal transplantation, and we found that about 5% of dead cells were present in the endothelial cells. It was considered that the increase of dead cells during storage might cause decrease of the number of the endothelial cells aftercorneal transplantation. In addition, when the rabbit cornea was stored at 4 ℃ in the corneal storage media, primary cilia in the corneal endothelial cells were disappeared, and they were reappeared after recovery culture at 37 ℃. The presence of primary cilia was thought to have some kind of relationship to viability of corneal endothelium.