Research on regenerative medicine of pediatric airways with functional laminar structure .

取兔耳软骨细胞，培养成软骨细胞片。我们用体外滚筒培养的方法将该片材制成圆柱形软骨结构。此外，我们还制作了具有不同功能的成纤维细胞层片和柱状纤毛上皮层片。我们尝试将这三种支架(从内到外依次放置柱状纤毛上皮层、软骨细胞层和成纤维细胞层)层叠，并尝试制作圆柱形功能结构，但导致再生失败。如果通过这种方法能够实现具有功能性层状结构的气管再生，那么使用再生气管将开发一种治疗儿童呼吸道疾病的新方法。

Auricular chondrocytes were harvested from rabbits and cultivated to form a chondrocyte sheet. We manufactured cylindrical cartilage structure from the sheet by the in vitro roller tube culture. Furthermore, we made the fibroblast layer sheet and column cilia epithelial layer sheet with the different function that varied in each. We tried laminating these three kinds of seats (We placed column cilia epithelial layers, a cartilage cell layer and the fibroblast layer sequentially from the inside), and tried to manufacture cylindrical functional structure, however resulted in failure of regeneration. If tracheal regeneration having functional laminar structure is enabled by this method, a new therapy for pediatric airway disease will be developed using the regeneration trachea.