Preparation of synaptosome derived from specified neural circuit

大脑皮层由各种类型的神经细胞组成，它们相互连接并构成复杂的网络。因此，有必要检查功能分子在特定神经连接处的行为，以了解大脑皮层的功能，发育和可塑性。本课题的目的是制备预先荧光标记的神经连接突触体样品。为此，我通过注射荧光示踪剂标记了视觉皮层中的神经元，从标记的组织中制备突触体样本，并根据其荧光强度使用流式细胞术对其进行分类。从注射了碳菁染料DiI的皮层组织中获得了成功标记的突触体。

The cerebral cortex consists of various types of neural cells which are interconnected and make up a complex network. Therefore, it is necessary to examine the behavior of functional molecules at specified neural connections to understand the function, development and plasticity of the cerebral cortex. The aim of the present project is to prepare the synaptosome sample derived from the neural connections that was fluorescently labeled in advance. To this end, I labeled the neurons in the visual cortex by injecting fluorescent tracers, prepared synaptosome samples from the labeled tissue, and sorted them using flowcytometry according to their fluorescent intensity. I could obtained the successfully labeled synaptosomes from cortical tissue injected with a carbocyanine dye DiI.