Construction of nerve cell body on a 3-dimensional culture device

本研究旨在开发一种用于构建神经样细胞三维细胞组合体的装置。用于细胞图案化的装置已经能够大量地产生大小可控的细胞组件，并同时评估它们在各种培养条件下的功能。对于原代细胞、组织来源干细胞和诱导的多能干细胞(IPS)，组织的大小都得到了控制。该装置上的细胞组件具有药物反应，蛋白质水平分析揭示了它们的一些功能。神经样细胞的2D培养也表明，该装置上产生了类神经网络，并改变了对电刺激神经的循环反应。

This study aims to develop a device for the construction of three dimensional cell assemblies of nerve-like cells. The device for cell patterning has enabled to generate cell assemblies with a controlled size in large numbers and to simultaneously evaluate their functions under various culture conditions. The size of the tissues was controlled for primary cells, tissue-derived stem cells, and induced plulipotent stem(iPS) cells. The cell assemblies on the device had their drug response, and protein level analysis revealed their some functions. The 2D culture of nerve-like cells also showed that a neural-like network was generated on the device and cyclic response to electric nerve stimulation was changed.