Development of analysis method of methylation level using Zn finger protein

我们的目的是开发利用甲基化DNA结合蛋白（MBD）和锌指蛋白融合荧光素酶分析DNA甲基化水平的方法（Zif-荧光素酶），并进行如下研究; 1）建立了一种利用MBD和Zif荧光素酶分析DNA甲基化水平的新方法。3）成功地进行了雄激素受体基因区DNA甲基化水平的分析。我们还成功地分析了抑制癌变的p16基因区域的DNA甲基化水平。因此，我们可以说，我们的方法有望广泛应用于各种目标基因区域的DNA甲基化分析。

We aimed to develop the method to analyze the DNA methylation level using Methylated DNA Binding protein(MBD) and Zinc finger protein fused luciferase(Zif-luciferase) and carried out the research as follows;1) We developed the novel method for analyzing the DNA methylation level using MBD and Zif-luciferase.2) We designed and constructed the novel Zif-luciferase which enable us the analysis of DNA methylation level of the gene region of androgen receptor which relates to the canceration.3) We succeed in the analysis of the DNA methylation level Zif-luciferase. We also succeed in the analysis of the DNA methylation level of the p16 gene region which suppressing canceration. Therefore we can say our method is expected for broad application of the DNA methylation analysis of various target gene region.