Identification of new determinants for double fertilization

获得了用荧光蛋白(GFP)特异显示配子膜的拟南芥标记系。用抗GFP抗体通过下拉策略从卵细胞膜标记系雌蕊组织中纯化了膜蛋白。在已鉴定的具有跨膜区的蛋白质中，发现一个与种子育性有关的蛋白质。

Arabidopsis marker lines, where gamete membrane was specifically visualized with fluorescent protein (GFP), were produced. Membrane proteins were purified from pistil tissue of the egg cell membrane- marker line by pull-down strategy using anti-GFP antibody. Among the identified proteins with putative trans-membrane region, one protein was found to be involved in seed fertility.