Development of a novel genome mapping method by using homologous recombination protein

为了标记细胞中的某个基因组区域，需要一个苛刻的条件来变性DNA。在这个方案中，我们试图使用一些同源重组蛋白将荧光标记的DNA序列靶向到基因组DNA。然而，使用同源重组蛋白RecA或XRad51，我们没有在固定的细胞中得到有效的靶向。作为替代，我们设计了一些能够识别特定DNA序列的荧光聚酰胺，并成功地靶向了固定细胞中的端粒重复序列

To label a certain genomic region in the cell, a harsh condition to denature DNA is required. In this proposal, we tried to target a fluorescently tagged DNA sequence to the genomic DNA using some homologous recombination proteins. However, with homologous recombination protein RecA or XRad51, we did not get an efficient targeting in the fixed cells. As an alternative, we designed some fluorescent polyamides, which can recognize a certain DNA sequence, and succeeded to target efficiently the telomere repeats in the fixed cells