Development and analysis of a tagged antibody producing mouse for the creation of a new antibody array technology

抗体芯片是一种用来详尽分析蛋白质表达的工具。这项研究的目的是通过开发一种高灵敏度的新一代抗体芯片，利用标记蛋白固定在钻石状表面处理的底物上的技术，创建一种新的抗体阵列技术。因此，我们利用基因重组技术将标签序列添加到免疫球蛋白G(Ig G)中，从而创造了一种单抗。在细胞培养系统中产生标签引入的免疫球蛋白和对照免疫球蛋白，抗体从它们的条件培养液中分泌和纯化。然后，将它们固定在底物上，以评估有或没有标签的底物上抗体的状态。结果，证实了大量高纯度的标记抗体是创建抗体阵列所必需的。因此，我们开发了一套高通量的标记抗体生产系统，为抗体阵列新技术的开发提供了有力的工具。

An antibody chip is a tool used to exhaustively analyze protein expression in full detail. The purpose of this study is to create a new technology for antibody arrays by developing a highly sensitive next generation antibody chip, using the technology where a tagged protein is immobilized on a diamond-like surface treated substrate. Thus, we created a monoclonal antibody where the tag-sequence was added to immunoglobulin G (IgG) using genetic recombination technology. The tag introduced IgG and a control IgG were produced in a cell culture system, where the antibodies were secreted and purified from their conditioned medium. Then, they were fixed to the substrate to evaluate the state of the antibody on the substrate with or without the tag. As a result, it was confirmed that a high purity of tagged-antibodies are necessary in large quantities for the creation of the antibody array. Therefore, a high-throughput production system of the tagged-antibody was developed, which would be a powerful tool in creating a new technology for antibody arrays.