Neural differentiation of human induced pluripotent stem cells by temperature-sensitive Sendai virus vectors

仙台病毒(Sendai Virus，SeV)载体可以有效地导入外源基因，而不需要将转基因基因插入各种器官，有望在临床上应用。我们通过在SeV主干中引入温度敏感突变来改进SeV载体，以便在不允许的温度下很容易地移除载体。我们通过在诱导多能干细胞(IPSCs)中表达Genex获得了产生多巴胺的神经元。这些载体使IPSCs能够高效地产生分化的细胞。

Sendai virus(SeV) vectors can efficiently introduce foreign genes without genomic insertion of transgenes into various organs and are expected to be clinically applicable. We improved the SeV vectors by introducing temperature-sensitive mutations into the SeV backbone so that the vectors could be easily removed at nonpermissive temperatures. We obtained dopamine-producing neurons by geneX expression in induced pluripotent stem cells(iPSCs). These vectors enabled the efficient generation of differentiated cells from iPSCs.