An experimental study on the development of new therapy for treatment of diabetes mellitus by using the combination of electrical stimulation and heat-stress

本研究旨在探讨电刺激(ES)前治疗性热应激对改善胰岛素抵抗的影响。在体外实验中，利用培养的肌肉细胞(C2C12肌管)，ES和热应激都增加了胰岛素刺激的葡萄糖摄取，但这种作用不是相加的。在体内实验中，通过使用高脂饮食诱导的胰岛素抵抗大鼠，每天应用ES改善了胰岛素反应，并增加了股直肌热休克蛋白(HSP)72和HSP27的表达。这些结果提示，日常应用ES可以改善胰岛素敏感性，其机制与心肌细胞内HSPs的表达有关。

The aim of this study was to investigate the effects of therapeutic heat-stress before electrical stimulation(ES) on improvement of insulin resistance. In vitro experiments by using cultured muscle cells(C2C12 myotubes), both ES and heat-stress increased insulin-stimulated glucose uptake, but the effects were not additive. In vivo experiments by using high-fat diet-induced insulin-resistant rats, the daily application of ES improved insulin responses and increased heat shock protein(HSP) 72 and HSP27 expression in the rectus femoris muscle. These results suggest that the daily application of ES can improve insulin sensitivity, and this mechanism is related to the expression of HSPs in myocytes.