Establishment of human induced cancer stem cells(iCSCs) and malignant brain tumor models derived from neural stem cells

神经干细胞具有自我更新能力和多潜能，被认为是脑肿瘤的起源细胞之一。转导有成分激活的ALK的神经干细胞迅速形成高增殖和侵袭性的脑肿瘤。这些肿瘤的组织学特征类似于人类麦芽状胶质母细胞瘤(GBM)，如坏死、血管周围袖带和巨细胞形成。免疫组织化学分析显示，这些肿瘤均为胶质瘤标志物和增殖标志物阳性。这些模型对于研究胶质瘤发生的分子机制是有用的。

Neural Stem Cells(NSCs) having self-renewal ability and multipotency are considered as one of the cells-of-origin of Brain tumors. The NSCs transduced with constitutively activated ALK rapidly formed highly proliferative and invasive brain tumors. Histological features of these tumors are similar to those of human glioblastoma maltiforme(GBM) such as necrosis, perivascular cuffing and giant cell formation. Immunohistochemistry analysis revealed that these tumors are positive for glioma markers and proliferation marker. These models are useful for investigating molecular mechanisms of gliomagenesis.