Oxidative stress signal function in stem cells via keap1-Nrf2 system

新的胚胎干细胞系是由Nrf2、Keap1缺失的胚细胞生成的。这些Nrf2 KO和Keap1 KO ES细胞预计在氧化应激反应中具有表型。从杂交杂合突变小鼠中获得Nrf2和Keap1缺失胚细胞。基因组PCR用于确定已建立的胚胎干细胞的基因型。Nrf2和Keap1缺失的ES细胞表现出典型的ES样形态。胚胎干细胞细胞系也显示出胚胎干细胞标记物的表达模式。胚胎体形成是胚胎干细胞的分化系统。

New ES cells lines are generated from Nrf2, Keap1 null blastocytes. These Nrf2 KO and Keap1 KO ES cells are expected to have phenotypes in oxidative stress responses. Nrf2 and Keap1 null blastocytes are obtained from cross mating heterozygous mutant mice. Genomic PCR are used to determine the genotypes of established ES cells. Nrf2 and Keap1 null ES cells show typical ES-like morphologies. The ES cell lines also show expression pattern of ES cell markers. Embryoid body formation are used as ES differentiation system.