Establishment of Glioma stem-like cells(GSC) from glioma patient tumors and analysis of the novel molecular markers of GSC by an integrated proteomics.

胶质瘤干细胞（GSCs）被认为是恶性胶质瘤治疗抵抗和复发的原因。调节这些细胞维持/分化的分子被认为是有效的治疗靶点。在本研究中，我们从恶性胶质瘤患者的肿瘤中建立了GSC克隆，并对其进行了整合蛋白质组学研究，以研究血清刺激诱导的GSC中差异表达的蛋白质和mrna。结果鉴定出30000个分子，氧化石墨烯分析显示，在GSC分化过程中，细胞粘附分子（包括整合素亚家族成员和细胞外基质）的表达显著上调。经过细胞生物学验证，我们发现血清因子诱导的ecm与分化和增殖的耦合。这些结果提出了GSC自身诱导/分泌ecm形成特定微环境的可能性，称为“分化生态位”，促进恶性胶质瘤的发展。

Glioma stem cells(GSCs) are thought to be responsible for the therapeutic resistance and recurrence of malignant glioma. The molecules regulating the maintenance/differentiation of these cells are considered effective therapeutic targets. In this study, we established GSC clones from tumors of malignant glioma patients and subjected them to integrated proteomics to survey the proteins and mRNAs differentially expressed in GSCs after they have been induced by serum stimulation. This resulted in the identification of 30000 molecules and GO analysis revealed that the expression of cell adhesion molecules, including integrin subfamily members and extracellular matrices(ECMs) was significantly upregulated during GSC differentiation. After the cell-biological validations, we found that serum factor-induced coupling of ECMs to differentiation and proliferation. The results raised the possibility that GSC induces/secretes ECMs by itself to form a specific microenvironment, called the "differention niche", which facilitates the development of malignant glioma.