Effective Production of Disease-Related Oligosaccharides and Preparation of Medical Devices

建立了一种利用细胞酶和供体底物生产与病毒或致病蛋白相互作用的寡糖的方法。所得低聚糖用于医疗器械的试制。利用四苯乙烯在高浓度下产生荧光，将寡糖与四苯乙烯结合，制备了两种分别检测人流感病毒和禽流感病毒的分子。此外，利用细胞学分子监测细胞内寡糖合成，寻找控制寡糖合成的药物。

A method to produce oligosaccharides, which interact with viruses or pathogenic proteins, by using cellular enzymes and donor substrate was established. The obtained oligosaccharides were used for the trial manufacture of the medical devices. Two kinds of molecules, which can detect human and avian influenza viruses, respectively, were developed by binding the oligosaccharides to the tetraphenylethylene which gives the fluorescence in high concentration. In addition, using the cytology molecules which could monitor intracellular oligosaccharide synthesis, searching the drug which could control oligosaccharide synthesis was attempted.