Low environmental load technology for liquid livestock waste based on control of novel reactions mediated by microorganisms

本研究探讨了利用饲草稻田中的反硝化甲烷氧化菌作为畜禽粪便充气肥料的可行性。设计了一种检测和定量检测这些细菌的聚合酶链式反应引物，阐明了它们在不同水稻土壤中的丰度和位置。一项基于水控制和液体牲畜粪便投入量的新战略使饲用稻田的温室气体--甲烷和一氧化二氮--显著减少。此外，还阐明了稻田排水后突然产生一氧化二氮的可能机制。

This study explored feasibility of harnessing denitrifying methane-oxidizing bacteria in a forage rice field as a site where aerated liquid livestock waste is supplied as a fertilizer. A PCR primer to detect and quantify these bacteria was designed, elucidating their abundances and locations in various rice paddy soils. A new strategy based on water control and the degree of liquid livestock waste dose allowed noticeable reductions of greenhouse gases, i.e. methane and nitrous oxide, from a forage rice paddy field. Furthermore, a plausible mechanism of abrupt nitrous oxide production from a rice paddy field after water drainage was elucidated.