ADF-STEM Analysis of Magnetic Particles in Bio-system without Staining

在外界粒子进入生物系统的转移机制中，识别途径和最终积累位点对于理解粒子与生物系统的相互作用至关重要。本研究的目的是提出一种高分辨率的生物样品电子显微镜分析方法，以实现粒子转移的直接空间分布分析。在本研究中，我们提出了扫描透射电子显微镜暗场法来分析以生物细胞等轻元素为主的标本，我们提出了不染色和厚样分析。因此，可以通过不染色的电子显微镜来分析引入磁性颗粒的细胞样品中微粒的定位。

In the transfer mechanism of external particles into the biological system, the identification of pathways and final accumulation sites is essential for the understanding of the interaction of particles and biological systems. The aim of this study was to propose a methodology for high-resolution electron microscopic analysis of biological samples for realizing the direct spatial distribution analysis of the particle transfer.In this study, a scanning transmission electron microscope dark field method was proposed for the analysis of a specimen mainly composed of light elements such as biological cells, by which we proposed a non-staining and also thick sample analysis. As a result, it was possible to analyze the localization of microparticles in cell samples introduced with magnetic particles by means of the non-staining electron microscopy,.