Modification of Conducting Polymer Films by Enzyme Immobilization for Fabrication of Electrodes of Biological Fuel Cells

利用导电聚合物薄膜制备生物电极，目的是将其应用于葡萄糖驱动的生物燃料电池。将葡萄糖氧化酶（GOx）共价固定在3-甲基噻吩（3MT）和噻吩-3-乙酸（T3A）的共聚物膜上，制备了一种酶电极，在电子转移介质存在下，该电极作为燃料电池的阳极表现出良好的性能。将漆酶（Lac）吸附在聚3mt薄膜上，制备了一种不含介质的直接电子转移型阴极。用GOx-3MT/T3A共聚物膜（阳极）和Lac-poly3MT膜（阴极）构建燃料电池，并利用植物纤维素酶解得到的葡萄糖溶液发电。由植物源性葡萄糖溶液驱动的燃料电池产生了相当大的功率输出（约10微米/平方厘米）。

Bioelectrodes were fabricated with conducting polymer films for the purpose of applying them to a biological fuel cell driven with glucose. An enzyme electrode was prepared by covalently immobilizing glucose oxidase (GOx) on a copolymer film of 3-methylthiophene (3MT) and thiophene-3-acetic acid (T3A), which showed a good performance as an anode of the fuel cell in the presence of an electron-transferring mediator. A cathode was prepared by adsorbing laccase (Lac) on poly3MT film, which functioned as a direct electron transfer type cathode without any mediator. A fuel cell was constructed with the GOx-3MT/T3A copolymer film (anode) and the Lac-poly3MT film (cathode) and subjected to electric power generation using glucose solutions obtained by enzymatic decomposition of plants cellulose. A considerable power output (ca. 10 microW/cm2-cathode) was generated by the fuel cell driven with the plants-derived glucose solutions.